Table 1: Solutions.

	Chemical Name	Final Concentration [mmol/L]	Mass to add for Suggested volume [g]
Magnesium Chloride Stock	Magnesium Chloride	500	10.267
0.1 L
	Chemical	Final Concentration [mmol/L]	Mass to add for Suggested volume [g]
Calcium Chloride Stock	Calcium Chloride	100	7.3505
0.5 L
	Chemical	Final Concentration [mmol/L]	Mass to add for Suggested volume [g]
Perfusion Stock	Sodium Chloride	113	6.604
1 L	Potassium Chloride	4.7	0.350
	Potassium Phosphate Monobasic	0.6	0.082
	Sodium Phosphate Dibasic	0.6	0.085
	Magnesium Sulfate	1.2	0.144
	Sodium Bicarbonate	12	1.008
	Potassium Bicarbonate	10	1.001
	4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid	10	2.383
	Taurine	30	3.755
	ultrapure water			q.s. to volume
	Chemical	Final Concentration [mmol/L]	Mass to add for Suggested volume [g]	Volume to add for suggested volume [mL]
Modified Tyrodes Stock	Sodium Chloride	140	8.223
1 L	Potassium Chloride	5.4	0.407
	4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid	10	2.395
	100 mmol/L Calcium Chloride Stock	1.8		18
	500 mmol/L Magnesium Chloride Stock	1		2
	ultrapure water			q.s. to volume
	Chemical	Final Concentration [W/V]	Mass to add for Suggested volume [g]	Volume to add for suggested volume [mL]
10% BSA stock	Albumin, Bovine Serum	10%	5.000
0.05 L	ultrapure water or Perfusion Solution Stock			q.s. to volume
	Chemical	Final Concentration [mmol/L]	Mass to add for Suggested volume [g]	Volume to add for suggested volume [mL]
Perfusion Solution	Perfusion Solution Stock			250
0.25 L	2,3-butanedione monoxime	10	0.258
	Glucose	0.10%	0.25
Notes:	Oxygenate to saturation of 10–20 ppm
	pH adjustment typically not needed
	Keep on ice
	Chemical	Final Concentration [mmol/L]	Mass to add for Suggested volume [g]	Volume to add for suggested volume [mL]
Modified Tyrodes Solution	Modified Tyrodes Stock			250
0.25 L	Glucose	10	0.45
Notes:	Oxygenate to saturation of 10–20 ppm
	adjust pH, typically ~180 uL of 5N NaOH for 25 C