|
|
|
|
| Bovine Serum Albumin |
Fisher BioReagents |
BP-1600 |
to coat tips of fine forcepts, scissors |
| Crile Hemostat |
Fine Science Tools |
13005-14 |
for mouse gross dissection |
| Rochester-pean Hemostat |
World Precision Instruments |
501708 |
for rat gross dissection |
| Metzenbaum Scissors |
Fine Science Tools |
14116-14 |
for mouse gross dissection |
| Mayo Scissors |
Fine Science Tools |
14110-15 |
for rat gross dissection |
| Iris Forceps |
World Precision Instruments |
15915 |
for removal of heart |
| Fine Scissors, curved |
Fine Science Tools |
14061-09 |
for removal of heart |
| Dumont #3 Forceps |
Fine Science Tools |
11231-30 |
2x for cannulation of aorta |
| Silk Suture, Size: 4/0 |
Fine Science Tools |
18020-40 |
cut to ~1.5 inch pieces, soaked in water |
| 23 gauge blunted needle/canula |
|
|
for cannulation of mouse aorta, use 1mm of PE50 tubing as stop |
| 18 or 16 gauge blunted needle/canula |
|
|
for cannulation of rat aorta, use 1mm of PE160 or PE205 tubing as stop |
| 5 mL syringe |
BD Luer-Lock |
309646 |
|
| Dumont #5 Forceps |
Fine Science Tools |
11254-20 |
2x for trabecula isolation |
| Student Vannas Spring Scissors |
Fine Science Tools |
91500-09 |
for opening of the RV |
| Vannas Spring Scissors |
Fine Science Tools |
15000-00 |
for trabecula isolation |
| Sodium Chloride |
Sigma-Aldrich |
S9888-1KG |
|
| Potassium Chloride |
Fisher Chemical |
P217-500 |
|
| Potassium Phosphate Monobasic |
Sigma-Aldrich |
795488-500G |
|
| Sodium Phosphate Dibasic |
Sigma-Aldrich |
S7907-100G |
|
| Magnesium Sulfate |
Sigma-Aldrich |
M7506-500G |
|
| Sodium Bicarbonate |
Sigma-Aldrich |
S6297-250G |
|
| Potassium Bicarbonate |
Sigma-Aldrich |
237205-100G |
|
| HEPES |
Sigma-Aldrich |
H3375-250G |
|
| Taurine |
Sigma-Aldrich |
T0625-1KG |
|
| D-(+)-Glucose |
Sigma-Aldrich |
G8270-1KG |
|
| 2,3-Butanedione Monoxime |
Sigma-Aldrich |
B0753-25G |
|
| Calcium Chloride Dihydrate |
Fisher Chemical |
C79-500 |
|
| Magnesium Chloride Hexahydrate |
Sigma-Aldrich |
M2670-100G |
|
| Sodium Hydroxide |
Sigma-Aldrich |
S8045-500G |
|
| Anethesia system |
EZ Systems |
EZ-SA800 |
Can use any appropriate anethesia method/system |
| Experimental system |
Aurora Scientific |
801C |
Can use any appropriate experimental chamber with force and length control |
| Data acquisition system |
MicrostarLabs |
DAP5216a |
Can use any DAQ. This is a PCI based data acqusition for use with SLControl; must have a PC with a PCI slot |
| Microscope/digital imaging system |
Olympus |
IX-73 |
Can use any appropriate microscope. Needed to measure muscle length, cross sectional area |
| Mounting Pin/Needle |
BD PrecisionGlide |
305136 |
For holding heart to dish. 27 G x 1-1/4 |
| Mounting Pin/Needle |
Fine Science Tools |
26000-40 |
For holding heart to dish. 0.4mm diameter insect pin (Alt to 27G needle) |
| Transfer Pipette |
FisherBrand |
13-711-7M |
cut ~1" from tip to widen bore |
| Syringe Holder |
Harbor Frieght |
Helping Hands 60501 |
Can be used as alternate for ring stand |
| Peristaltic Pump |
Rainin |
Rabbit |
Can be any means to create flow in experimental chamber |
| Containers/dissection dishes |
FisherBrand |
08-732-113 |
Weigh dishes for creating dissection plates |
| SYLGARD 184 Silicone Elastomer Base |
Dow Corning |
3097358-1004 |
For creating dissection plates |
| Isoflurane |
VetOne |
502017 |
|
| Oxygen (O2) |
AirGas |
OX USP300 |
|
| 95% Oxygen/5% CO2 |
AirGas |
Z02OX9522000043 |
|
| Data acquisition software |
SLControl |
|
|
| Data analysis software |
Mathworks |
Matlab |
Custom Script |
| pH and Oxygen sensor |
Mettler Toledo |
SevenGo pH and DO |
|
| Microscope connected camera |
Flir |
BFS-U3-27S5M-C |
Includes acquisition software |
| Imaging software |
IrfanView |
|
|
| Stereomicroscope |
AmScope |
SM-1TX |
|
| Gooseneck Piggyback Illuminator |
AmScope |
LED-6WA |
|
