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. 2023 Nov 8;17(22):22901–22915. doi: 10.1021/acsnano.3c08030

Figure 2.

Figure 2

Dgcr8-deficient intestinal epithelium fails to regenerate after 5-FU induced injury. (A) Schematic diagram showing the 5-FU treatment in WT and Dgcr8 KO mice. (B,C) Body weight change and Kaplan–Meier survival curve of WT and Dgcr8 KO mice after 5-FU administration. n = 10 biological replicates for each genotype. (D) Histological images of small intestines (left) and percentage of surviving crypts (right) from WT and Dgcr8 KO mice at indicated time points after 5-FU administration. Images are representative of at least 3 mice at each time point for each genotype. (E,F) IF staining (left) and quantification (right) of Olfm4+ ISCs (E) and Ki67+ TA cells (F) in the small intestinal epithelium from WT and Dgcr8 KO mice. Epithelial cells were stained by E-cadherin (red). n ≥ 3 biological replicates for each genotype. (G–I) IF staining for CD45, IFNγ, and phosphorylated NF-κB (left) and quantification of CD45, IFNγ, and phosphorylated NF-κB positive cells (right) in intestinal epithelia from WT and Dgcr8 KO mice 3 days after 5-FU administration. n ≥ 3 biological replicates for each genotype. Statistics data represent mean with SD. p-values were generated by unpaired two-tailed Student’s t-test (B, D–I) and one-sided log-rank test (C). **p < 0.01; ***p < 0.001; ****p < 0.0001. Scale bars: 100 μm (D–I).