FIG. 2.
IKe gene-lacZ fusions used to measure translation of genes V and VII. The IKe sequences shown are as follows: pSM5, 103 bp upstream from gene V ATG and 15 codons; pSM7, 89 bp before the first in-frame ATG for gene VII and 5 additional triplets; pSM5-7, 110 bp upstream from the gene V ATG, gene V, and the gene VII site-lacZ fusion present in pSM7. Transcription from these and all other lacZ fusions reported was directed by the lacIq promoter in parent vector pKC8. pKC8 contains the lacIq promoter and 11 bp of lacI sequence downstream from the transcription start site, followed by restriction sites (EcoRI, SmaI, and BamHI) and the lacZ coding region starting at codon 9 (8). lacI and lacZ sequences in the vector (continuous lines), the IKe gene sequences (open bars), and initiator regions (shaded bars; with ATG position indicated by a vertical line) are shown.