TABLE 2.
Plasmids
| Plasmid(s) | Description | Source or reference |
|---|---|---|
| pKC8 | pMC1403 with lacIq promoter in EcoRI site upstream from lac sequences | 8 |
| pSM5 | pKC8 with IKe gene V initiation site and first 15 codons in frame with lacZ coding region | This work |
| pSM7 | pKC8 with the IKe gene VII initiation site and first 4 codons in frame with the lacZ coding region | This work |
| pSM5-7 | pKC8 with IKe gene VII-lacZ fusion as in pSM7, but preceded by IKe gene V | This work |
| pSM7AC, pSM7CA, pSM7CC | pSM7 with second or first ATG or both changed to CTG | This work |
| pSM5-7AC, pSM5-7CA, pSM5-7CC | pSM5-7 with second or first ATG or both changed to CTG | This work |
| pSM5-7TAG, pSM5-7TAA | pSM5-7 with gene V TGA changed to TAG or TAA | This work |
| pSM5-7CAG | pSM5-7 with out-of-frame GTG in gene VII changed to CAG | This work |
| pGI73 | pSP73 with 335-bp Sau3A/Csp451 fragment from IKe (nt 1404 to 1738) inserted downstream from SP6 promoter | 49 |
| pSM7.87, etc.a | pSM7 deletion derivatives with the indicated number of nucleotides remaining upstream from gene VII initiator ATG | This work |
| pSM5-7TGG | pSM5-7 with gene V stop codon (TGA) changed to TGG | This work |
| pSMΔ5-7 | pSM5-7 with 135-nt in-frame internal deletion in gene V | This work |
| pSMΔ5-7 +54, +15, −2 | pSMΔ5-7 with the distal portion of gene V in alternate reading frames to place a stop codon at the indicated positions before or after gene VII initiator ATG | This work |
| pM506, pM565, pM563, pJ32 | M13 vectors carrying lacZ coding region segments | 25 |
a
For details on these plasmids, see the legend to Fig. 6.