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Procedure for gating monocytes by flow cytometry. Monocytes were identified from peripheral blood mononucelar cells with CD45 and CD33 expression after successive exclusion of T lymphocytes, natural killer (NK) cells, residual lymphocytes and basophils. Then monocytes were analyzed regarding CD14/CD16 expression for classical, intermediate and non-classical monocytes. KO: knockout; SSC: side scatter.
