Table 2.

Primer information used in this study.

Primer	Sequence (5'-3')	Tm (°C)	Purpose
MT-F16 a	CGACGTCTTCGCCTACCC	57.4	Amplifying ALS gene fragments covering Ala-122, Pro-197, Ala-205, and Phe-206
MT-R16 a	AGCCATCTGCTGTTGGATGT	53.8
ALS-F5	CACAACTACCTGGTCCTCG	55.5	Amplifying ALS gene fragments covering Asp-376, Arg-377
ALS-R5	AGAAGATCATTCATGCCCT	49.0
MT-B1 b	GTGGATAAGGCCGACCTGTTGCT	62.3	Amplifying ALS gene fragments covering Trp-574, Ser-653, Gly-654
MT-B2 b	CTGCCATCACC(A/G)TCCAGGATCAT	60.7
ALS-qF1	GTGTATGCAAATTATGCGGTGGA	59.9	qPCR
ALS-qR1	ACCAATCTCAGCTGGATCAATGT	60.0
UBQ-qF1	CTCACCGGGAAGACTATCACC	59.6	qPCR normalisation
UBQ-qR1	ATGCCCTCCTTGTCTTGAATCTT	60.0
Actin-qF1	GTTATCACAATCGGTGCAGAGAG	59.5	qPCR normalisation
Actin-qR1	AACATAGTAGAGCCACCACTGAG	59.5
18S rRNA-qF3	TCTGCTCGCTGATCCGATGAT	61.4	qPCR normalisation
18S rRNA-qR3	TCAGGCTCCCTCTCCGGAAT	62.2
GAPDH-qF1	GCGTCAACGAGAAGGAATACAAG	59.9	qPCR normalisation
GAPDH-qR1	CCCATCAACAGTCTTCTGGGTAG	60.4

^aSource from Mei et al., 2017.

^bSource from Li et al., 2017.