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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: J Vasc Surg. 2020 Jan 21;72(3):1087–1096.e1. doi: 10.1016/j.jvs.2019.08.285

Fig 1.

Fig 1.

Treg proportion in the peripheral blood of abdominal aortic aneurysm (AAA) patients was significantly decreased compared with control patients. Human peripheral blood mononuclear cells were isolated from peripheral blood of AAA and control patients. Total RNA was extracted and messenger RNA levels of Foxp3 and CD4 genes were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR). A, Relative messenger RNA expression levels of Foxp3 to CD4 in human Peripheral blood mononuclear cells (PBMCs) from control (n = 52) and AAA (n = 48) patients. The ratio of Foxp3 to CD4 mRNA levels is shown. B, Percentage of Tregs (CD4+CD25+FoxP3+) in the peripheral blood of control (n = 12) and AAA (n = 10) patients determined by flow cytometry; representative dot plot (left). Data represented as mean 6 standard deviation, *P < .05.