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. 2023 Dec 1;21:872. doi: 10.1186/s12967-023-04742-y

Table 2.

Core Gene Pathway Alterations and Association with Lymph Nodes Involvement

Mutated pathway KRASmut KRASmut KRASmut KRASmut KRASWT
Overall, n=473 Tumor size ≤ 2cm, n=84 Tumor size (2, 3cm], n=211 Tumor size > 3cm/T4, n=178 Overall, n=40
N1-2 P N1-2 P N1-2 P N1-2 P N1-2 P
TP53
 WT 90/172 (52.3%) 1 8/34 (23.5%) 1 45/79 (57.0%) 1 37/59 (62.7%) 1 12/30 (25.0%) 1
 Missense 106/197 (53.8%) 0.776 17/31 (54.8%) 0.010 45/80 (56.3%) 0.928 44/86 (51.2%) 0.169 4/9 (40.4%) 0.812
 Truncating 46/93 (49.5%) 0.656 5/18 (27.8%) 0.736 23/46 (50.0%) 0.451 18/29 (62.1%) 0.953 1/1
 Others* 6/11 (54.5%) 0.886 1/1 4/6 1/4 None
 Mutated 158/301 (52.5%) 0.972 23/50 (46.0%) 0.036 72/132 (54.5%) 0.732 63/119 (52.9%) 0.216 5/10 (50.0%) 0.580
Cell cycle
 WT 202/387 (52.2%) 1 25/72 (34.7%) 1 92/170 (54.1%) 1 85/145 (58.6%) 1 15/37 (40.5%) 1
 Mutated 46/86 (53.5%) 0.828 6/12 (50.0%) 0.310 25/41 (61.0%) 0.428 15/33 (45.5%) 0.169 2/3 (66.7%) 0.565
TGFb
 WT 195/374 (52.1%) 1 26/72 (36.1%) 1 94/170 (55.3%) 1 75/132 (56.8%) 1 15/34 (44.1%) 1
 Mutated 53/99 (53.5%) 0.805 5/12 (41.7%) 0.712 23/41 (56.1%) 0.926 25/46 (54.3%) 0.771 2/6 (33.3%) 1.000
Mutated pathways
 0-1 180/344 (52.3%) 1 22/68 (32.4%) 1 84/152 (55.3%) 1 74/124 (59.7%) 1 16/35 (45.7%) 1
 2-3** 68/129 (52.7%) 0.940 9/16 (56.3%) 0.075 33/59 (55.9%) 0.930 26/54 (48.1%) 0.154 1/5 (20.0%) 0.373
Trithorax
 WT 202/374 (54.0%) 1 27/75 (36.0%) 1 100/166 (60.2%) 1 75/133 (56.4%) 1 10/29 (34.5%) 1
 Mutated 46/99 (46.5%) 0.181 4/9 (44.4%) 0.620 17/45 (37.8%) 0.007 25/45 (55.6%) 0.922 7/11 (63.6%) 0.096
WNT
 WT 232/430 (54.0%) 1 31/78 (39.7%) 1 106/187 (56.7%) 1 95/165 (57.6%) 1 17/38 (44.7%) 1
 Mutated 16/43 (37.2%) 0.036 0/6 11/24 (45.8%) 0.314 5/13 (38.5%) 0.181 0/2
Hedgehog
 WT 246/461 (53.4%) 1 31/81 (38.3%) 1 116/204 (56.9%) 1 99/176 (56.3%) 1 16/37 (43.2%) 1
 Mutated 2/12 (16.7%) 0.012 0/3 1/7 (14.3%) 0.046 1/2 (50.0%) 1.000 1/3 (33.3%) 1.000

*Other mutation subtypes included mixed cases, in-frame insertion or deletion and silent mutation

**Alterations of 2-3 pathways among TP53, cell cycle pathway and TGFb pathway; Chi-squared test; Fisher exact test