FIG. 1.

Derepression of cys-3. The derepression process was monitored by shifting wild-type N. crassa mycelia from medium with a repressing level of methionine (5 mM) to medium with a derepressing level of methionine (0.25 mM). Mycelia were harvested, and total RNA and protein extracts were prepared. The harvest time of each sample is indicated at the top. (A) RNA blot analysis of cys-3 transcript (1.6 kb) and β-tubulin (2.0 kb) control; (B) Western blot analysis of CYS3 protein, using rabbit anti-CYS3 antibodies; (C) time course of aryl sulfatase activity. OD₄₀₅, optical density at 405 nm.