Table 1.
Regression and validation parameters of the proposed HPLC and HPTLC methods for determination of MIC, NYS, and MET
| Parameters | HPLC | HPTLC | ||||
|---|---|---|---|---|---|---|
| MIC | NYS | MET | MIC | NYS | MET | |
| Linearity | 5–50 μg/mL | 4–50 μg/mL | 4–40 μg/mL | 0.4–2 μg/band | 0.4–2.2 μg/band | 0.4–2 μg/band |
| Slope | 13.9680 | 4.3515 | 28.2340 | 0.4762 | 0.8460 | 1.102 |
| Intercept | − 0.9486 | 193.2800 | 3.8488 | 0.2186 | − 0.2224 | 0.4583 |
| Correlation coefficient | 0.9999 | 0.9999 | 0.9999 | 0.9999 | 0.9998 | 0.9999 |
| Accuracy (%) | 100.41 | 100.42 | 99.19 | 99.53 | 100.28 | 100.00 |
| Repeatability (RSD%)a | 0.17 | 0.62 | 0.61 | 0.80 | 0.49 | 0.77 |
| Intermediate Precision (RSD%)b | 0.71 | 0.82 | 0.66 | 1.33 | 0.67 | 1.69 |
| LODc | 1.19 μg/mL | 0.95 μg/mL | 0.80 μg/mL | 0.11 μg/band | 0.09 μg/band | 0.07 μg/band |
| LOQc | 3.62 μg/mL | 2.87 μg/mL | 2.43 μg/mL | 0.34 μg/band | 0.27 μg/band | 0.22 μg/band |
aThe intraday precision (n = 3), average of three different concentrations repeated three times within day
bThe interday precision (n = 3), average of three different concentrations repeated three times in three successive days
cLimit of detection and quantitation are determined via calculations: LOD = (SD of the response/slope) × 3.3; LOQ = (SD of the response/slope) × 10