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. 2023 Dec 1;17(1):173. doi: 10.1186/s13065-023-01083-1

Table 2.

Quantitative determination of NYS and MET in Amrizole N® vaginal suppository and NYS and MIC in Monicure plus® vaginal suppository by the proposed HPLC and HPTLC methods and application of standard addition technique

Pharmaceutical formulation Drug HPLC HPTLC
Taken
(μg/mL)
Founda % ± SD Pure added
(μg/mL)
%Recoveryb Taken
(μg/band)
Founda % ± SD Pure added (μg/band) %Recoveryb

Amrizole N® vaginal suppository

Batch NO. (977,243)

NYS 40.00 0.57 ± 99.08 3.00 99.27 1.00 0.86 ± 99.11 0.60 100.00
6.00 98.42 0.80 101.11
9.00 98.96 1.00 99.60
SD ±  Mean  98.88 ± 0.43 SD ±  Mean  0.78 ±  100.24 
MET 12.00

 ± 102.55

0.44

6.00 98.39 1.00

±  102.32 

0.27

0.50 99.94
12.00 98.31 0.60 100.50
18.00 98.52 1.00 98.96
SD ±  Mean   0.11 ±  98.41 SD ±  Mean  0.78 ±  99.80 

Monicure plus®

vaginal suppository

Batch NO. (7,373,004)

NYS 40.00

± 101.28 

0.66

3.00 100.87 1.00

±  101.03 

0.44

0.60 100.00
6.00 100.13 0.80 100.38
9.00 100.40 1.00 100.16
SD ±  Mean  0.38 ±  100.47  SD ±  Mean  0.19 ± 100.18 
MIC 15.00

± 102.28 

0.33

10.00 100.16 1.00

±  102.03 

0.54

0.60 98.81
15.00 99.37 0.80 98.16
25.00 98.45 1.00 98.36
SD ±  Mean  0.86 ±  99.33 SD ±  Mean  0.33 ±  98.44 

aAverage of six experiments

bAverage of three experiments