Figure 1. CYP-induced upregulation of JMJD3 expression is significantly reversed by emodin, in vitro and in vivo. (a) The mouse bladders were harvested at the end of the experiment, comparison of gene expression of JMJD3 among all groups, by RT-qPCR. (b) JMJD3 expression was analyzed using immunofluorescence. Scale bar = 20 μm final magnification. (c) Quantitative analysis of immunofluorescence data. (d) hBSMC cell viability was assessed by CCK-8 assay, and the cell viability in intervention groups was not lower than that in control group. (e) Comparison of JMJD3 expression in cell samples among all groups, by RT-qPCR. (f) Western blot analysis and quantification of JMJD3 protein expression in different groups. Data are represented as the mean ± standard deviation.

Source: Elaborated by the authors. JMJD3: lysine-specific demethylase 6B; IC: interstitial cystitis; OD: optical density; hBSMC: human bladder smooth muscle cells; LPS: lipopolysaccharide; CYP: cyclophosphamide; RT-qPCR: real-time quantitative polymerase chain reaction; compared with control (black rectangle box), * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; compared with IC group, # P < 0.05, ## P < 0.01 represent significant differences between the indicated columns.