TABLE 1.
Total and Se-dependent glutathione peroxidase activity increased while total glutathione decreased following treatment with LoaOOHa
| LoaOOH concn (mM) | GPX activity (nmol min−1 mg−1)b
|
Total glutathionec | GSSG (nmol/107 cells) | GSH/GSSG ratio | |
|---|---|---|---|---|---|
| Total | Se dependent | ||||
| 0 | 123 ± 4.2 | 96 ± 10 | 4.3 | 0.56 | 6:1 |
| 0.05 | 149 | 139 ± 7.1 | 2.8 | 0.32 | 7:1 |
| 0.08 | 611 ± 22 | 419 ± 24 | 1.45 | 0.19 | 3:1 |
| 0.12 | 593 ± 18 | 562 ± 20 | 0.62 | 0.05 | 11:1 |
| 0.2 | ND | ND | 0.43 | 0.01 | 55:1 |
a
Abbreviations: GPX, glutathione peroxidase; GSSG, oxidized glutathione; GSH, reduced glutathione; ND, not determined.
b
Activity is expressed as nanomoles of NADPH oxidized per minute per milligram of protein. Data are means of duplicates from a representative experiment.
c
Expressed as nanomoles of GSH plus 2× GSSG per 107 viable cells for the wild-type strain.