Figure 1. Overview of Visual Pathway Tissue Preparation and Histology.

(A) Scheme of the anterior visual pathway including the right (OD), left eye (OS), and optic nerves with chiasm. Boxes indicate where the tissue was separated for histologic processing. (B) Representative image of a longitudinal optic nerve section (T cells, CD3 staining). Paraffin-embedded optic nerves including the chiasm were cut longitudinally into 5-μm thick sections. (C) Histologic representation of a transversely cut right eye including optic nerve head (hematoxylin-eosin staining). Paraffin-embedded bulbi were cut transversally into 7-μm thick sections including the optic nerve head. (D) Histologic representation of a retina flat mount prepared from fresh PFA-fixed left eyes (RGCs, Brn3a staining). PFA: paraformaldehyde; RGCs: retinal ganglion cells.