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. 2023 Jul 10;10(5):e200141. doi: 10.1212/NXI.0000000000200141

Figure 4. Investigation of NK Cell Involvement to Assess ADCC in the Optic Nerve.

Figure 4

(A) Disease model comparison of number of NKp46-positive cells (NK cells) in optic nerves at presymptomatic disease phase. One experiment. Kruskal-Wallis test. p = ns. (B) Disease model comparison of number of NK cells (left) and (C) percentage of NK cells, negative (green) and positive (red) for CD107a cell surface staining (activated NK cells, right) in optic nerves at acute disease phase. Two independent experiments. Kruskal-Wallis test. p = ns. (D) Histologic representation of NKp46 and CD107a IF staining (DAPI counterstain) at acute disease (Iso-IgG EAE animal). Arrows indicate NKp46-positive cells. Insets show a higher magnification of boxed NKp46/CD107a double-positive cell with overlay analysis performed in image J. Scale bars = 50 and 20 μm (insets). ADCC = antibody-dependent cellular cytotoxicity; AQP = aquaporin; DAPI = 49,6-diamidino-2-phenylindole; EAE = experimental autoimmune encephalomyelitis; IgG = immunoglobulin G; IF = immunofluorescence; Iso = isotype control; MOG = myelin oligodendrocyte glycoprotein; ROI = region of interest.