Skip to main content
. 2023 Dec 1;4(6):e439. doi: 10.1002/mco2.439

FIGURE 3.

FIGURE 3

USP22 exerts tumor‐promoting functions and drives rapamycin sensitivity in hepatocellular carcinoma cells. (A) The effects of USP22 knockdown or overexpression on cell proliferation in Huh‐7 and Hep‐3B cells detected using a cell counting kit‐8 (CCK‐8) assay (n = 4). (B) The sensitivities of USP22 knockdown or overexpression cells toward rapamycin assessed in Huh‐7 and Hep‐3B cells using a CCK‐8 assay (n = 4). (C) Variations in rapamycin sensitivity detected by trypan blue staining‐based cell counts (n = 3). (D) Colony formation results and rapamycin sensitivity variations of Huh‐7 and Hep‐3B cells after USP22 knockdown or overexpression visualized by crystal violet staining. (E) The corresponding quantization diagrams of (D) (n = 3). (F) Migratory properties and variations in rapamycin sensitivity of Huh‐7 and Hep‐3B cells after USP22 knockdown or overexpression visualized by crystal violet staining. (G) The corresponding quantization diagrams of (F) (n = 3) (* p < 0.05, ** p < 0.01, *** p < 0.001).