FIG. 3.

Sensitivities of gnt operon expression to CR under conditions of high (A) and low (B) levels of culture agitation. Cell growth conditions, crude extract preparation, and β-galactosidase assays were as described in Materials and Methods. β-Galactosidase specific activity (units per milligram of protein) is given on the y axis, while the strains are indicated on the x axis as follows: wild type, ST100; ccpA⁻, ST101; ccpB⁻, ST102; ccpA⁻ ccpB⁻, ST103; ptsH1, ST105. Open bars, LB medium; hatched bars, LB medium plus 1% gluconate; open bars with black dashes, LB medium plus 1% gluconate plus 1% glucose; stippled bars, LB medium plus 1% gluconate plus 1% mannitol. Error bars denote standard deviations.