TABLE 1.
B. subtilis strains used in this study
| Strain | Genotypea | Reference or sourceb |
|---|---|---|
| JH642 | trpC2 pheA1 | J. A. Hoch |
| JH12761 | trpC2 pheA1 Δ(spo0E) | 29 |
| GM1221 | trpC2 pheA1 Δ(bgalX) amyE::(gntRK′-′lacZ) (Camr) | 4 |
| GM1222 | trpC2 pheA1 Δ(bgalX) amyE::(gntRK′-′lacZ) ptsH1 (Camr) | 4 |
| WLN29 | aroG932 trpC2 ccpA::Tn917lac | 10 |
| MD164 | trpC2 amyE::(xylAp-CRE-spoVG-lacZ) | 2 |
| ST100 | trpC2 pheA1 Δ(bgalX) amyE::(gntRK′-′lacZ) Δ(spo0E) | JH12761 chromosomal DNA tf GM1221 |
| ST101 | trpC2 pheA1 Δ(bgalX) amyE::(gntRK′-′lacZ) Δ(spo0E) ccpA::Tn917lac | WLN29 chromosomal DNA tf ST100 |
| ST102 | trpC2 pheA1 Δ(bgalX) amyE::(gntRK′-′lacZ) Δ(spo0E) ccpB::Tn10 | This work |
| ST103 | trpC2 pheA1 Δ(bgalX) amyE::(gntRK′-′lacZ) Δ(spo0E) ccpA::Tn917lac ccpB::Tn10 | ST102 chromosomal DNA tf ST101 |
| ST104 | trpC2 pheA1 Δ(bgalX) Δ(spo0E) ccpA::Tn917lac | WLN29 chromosomal DNA tf ST100 |
| ST105 | trpC2 pheA1 Δ(bgalX) amyE::(gntRK′-′lacZ) Δ(spo0E) ptsH1 (Camr) | GM1222 chromosomal DNA tf St100 |
| ST121 | trpC2 pheA1 ccpA::Tn917lac | WLN29 chromosomal DNA tf JH642 |
| ST123 | trpC2 pheA1 ccpA::Tn917lac ccpB::Tn10 | ST102 chromosomal DNA tf ST121 |
| ST124 | trpC2 pheA1 ccpA::Tn917 | p917::Phl tf ST121 |
| ST125 | trpC2 pheA1 ccpA::Tn917 ccpB::Tn10 | p917::Phl tf ST123 |
| ST131 | trpC2 amyE::(xylAp-CRE-spoVG-lacZ) ccpA::Tn917 | ST124 chromosomal DNA tf MD164 |
| ST132 | trpC2 amyE::(xylAp-CRE-spoVG-lacZ) ccpB::Tn10 | ST125 chromosomal DNA tf MD164 |
| ST133 | trpC2 amyE::(xylAp-CRE-spoVG-lacZ) ccpA::Tn917 ccpB::Tn10 | ST125 chromosomal DNA tf MD164 |
| ST137 | trpC2 amyE::(xylAp-CRE-spoVG-lacZ) ccpB::pHT181 ccpA::Tn917 | pHT181 ΔccpB tf ST131 |
| ST138 | trpC2 amyE::(xylAp-CRE-spoVG-lacZ) orf126::pHT181 ccpA::Tn917 | pHT181 Δorf126 tf ST131 |
| ST139 | trpC2 amyE::(xylAp-CRE-spoVG-lacZ) orf184::pHT181 ccpA::Tn917 | pHT181 Δorf184 tf ST131 |
a
GM1221 and GM1222 contain a pJH101 derivative (conferring chloramphenicol [Cam] resistance) inserted downstream of ptsH1 in GM1222.
b
tf, the indicated chromosomal DNA was used to transform the indicated recipient strain.