ADAR1 Zα domain P195A mutation activates the MDA5-dependent RNA-sensing signaling pathway in brain without decreasing overall RNA editing

. Author manuscript; available in PMC: 2023 Dec 1.

Published in final edited form as: Cell Rep. 2023 Jul 6;42(7):112733. doi: 10.1016/j.celrep.2023.112733

KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
ADAR1 Antibody (15.8.6)	Santa Cruz Biotechnology, Inc.	Cat#: sc-73408
β-Actin Antibody (C4)	Santa Cruz Biotechnology, Inc.	Cat#: sc-47778
Critical commercial assays
MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel	Millipore Sigma	Cat#: MCYTOMAG-70K
MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel II	Millipore Sigma	Cat#: MECY2MAG-73K
Deposited data
RNA-seq data	NCBI Gene Expression Omnibus	accession ID GSE229884
Experimental models: Organisms/strains
Mouse:Adar^P195A/P195A	Guo et al. 2021²⁶	NA
Mouse:Adar^P195A/−	This paper	NA
Mouse:Adar ^+/−	Wang et al. 2004³⁶	NA
Mouse: Ifih1^−/−	The Jackson Laboratory	Stock#: 015812
Oligonucleotides
See Table S9.	This paper	N/A
Software and algorithms
Trimmomatic	Bolger et al. 2014⁵⁹	http://www.usadellab.org/cms/?page=trimmomatic
STAR	Dobin et al. 2013⁶⁰	https://github.com/alexdobin/STAR
bcftools	Li et al. 2009⁶¹	https://samtools.github.io/bcftools/bcftools.html
snpSift	Cingolani et al. 2012⁶²	https://pcingola.github.io/SnpEff/