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. 2005 Mar 22;33(5):e52. doi: 10.1093/nar/gni055

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Structure of pMODloxZeoΔAmp3. (A) The structure of pMODloxZeoΔAmp3, which serves a transposable element having loxP sequences and Zeocin-resistance marker. This plasmid does not confer ampicillin resistance because its bla gene is truncated, but does confer Zeocin resistance through EM7 promoter-Zeo cassette (B). The nucleotide sequence of the transposable segment is shown. The loxP sequences are indicated by solid boxes. The positions of sequencing primers, lox-Cla and lox-Nsi were indicated by arrows.