Table 3.

Number of unigenes encoding key enzymes involved in steroidal saponins biosynthesis in three Polygonatum plants.

Enzyme name	EC number	unigene number	DEGs Number
Acetyl-CoA C-acetyltransferase (AACT)	2.3.1.9	7	5
Hydroxymethylglutaryl CoA synthase (HMGS)	2.3.3.10	6	6
3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR)	1.1.1.34	10	9
Mevalonate kinase (MVK)	2.7.1.36	5	5
Phosphomevalonate kinase (PMVK)	2.7.4.2	4	3
Diphosphomevalonate decarboxylase (MVD)	4.1.1.33	4	2
1-deoxy-D-xylulose-5-phosphate synthase (DXS)	2.2.1.7	11	7
1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR)	1.1.1.267	2	2
2-C-methyl-d-erythritol 4-phosphate cytidylyltransferase (MCT)	2.7.7.60	2	2
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (CMK)	2.7.1.148	3	2
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MCS)	4.6.1.12	2	2
(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (HDS)	1.17.7.1	3	3
4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR)	1.17.7.4	3	3
Isopentenyl-diphosphate Delta-isomerase (IDI)	5.3.3.2	10	8
Farnesyl diphosphate synthase (FPPS)	2.5.1.1,2.5.1.10	3	2
Squalene synthase (SS)	2.5.1.21	9	8
Squalene epoxidase (SE)	1.14.14.17	6	5
Cycloartenol synthase (CAS)	5.4.99.8	2	2
Sterol 24-C-methyltransferase (SMT1)	2.1.1.41	3	2
Plant 4,4-dimethylsterol C-4 alpha-methyl-monooxygenase (SMO1)	1.14.18.10	9	5
Cycloeucalenol cycloisomerase (CPI1)	5.5.1.9	8	8
Delta 14-sterol reductase (FK)	1.3.1.70	5	5
Cholestenol Delta-isomerase (HDY1)	5.3.3.5	6	6
24-methylenesterol C-methyltransferase (SMT2)	2.1.1.143	10	10
Plant 4 alpha-monomethylsterol monooxygenase (SMO2)	1.14.18.11	5	4
Delta 7-sterol 5-desaturase (STE1)	1.14.19.20	8	6
7-dehydrocholesterol reductase (DWF5)	1.3.1.21	5	4
Delta 24-sterol reductase (DWF1)	1.3.1.72	4	2