Figure 6.

CD4⁺CD8⁺ T cells in the IEL compartment were microbiota dependent. (A) Relative abundance of the Lactobacillus genus extrapolated from 16S sequencing reads. Feces were collected at the time points of 0, 1, and 2 weeks of EAU (1, 2, and 3 weeks of ABX), and the compiled results are shown. *P < 0.05, **P < 0.01 by ordinary one-way ANOVA. (B) Quantification of L. reuteri by qPCR from fecal DNA (no treatment, n = 7; short-term ABX, n = 6; long-term ABX, n = 4). *P < 0.05 by two-way ANOVA relative to no treatment. (C) Linear regression analysis of CD4⁺, CD4⁺CD8⁺ (DP), or CD8⁺ IEL subsets and abundance of eubacteria, Bacteroidetes and Firmicutes phyla, or L. reuteri. P values shown in the graphs indicate the significant linear relationships between IEL subsets and bacterial abundance (DP, green; CD4, red). No significance was found in the CD8⁺ population.