TABLE 1.
Strains used in this study
| Strain | Genotypea | Reference or sourceb |
|---|---|---|
| B. subtilis | ||
| GM273 | trpC2 Δ(ptsGHI) sacB′-′lacZ | 5 |
| ST100 | trpC2 pheA1 Δ(bgalX) amyE::(gntRK′-′lacZ) Δ(spo0E) | 4 |
| ST141 | trpC2 pheA1 Δ(bgalX) amyE::(gntRK′-′lacZ) Δ(spo0E) glcP::Tn10 | This study |
| ST142 | trpC2 Δ(ptsGHI) sacB′-′lacZ glcP::Tn10 | ST141 chromosomal DNA tf GM273 |
| ST143 | trpC2 pheA1 Δ(bgalX) amyE::(gntRK′-′lacZ) Δ(spo0E) ptsG::Tn10 | This study |
| ST144 | trpC2 pheA1 Δ(bgalX) amyE::(gntRK′-′lacZ) Δ(spo0E) ptsG::Tn10 | This study |
| ST145 | trpC2 pheA1 Δ(bgalX) amyE::(gntRK′-′lacZ) Δ(spo0E) glcT::Tn10 | This study |
| E. coli | ||
| INVαF′ | F′ endA1 recA1 hsdR17 (rK− mK+) supE44 thi-1 gyrA96 relA1 φ80lacZΔM15 Δ(lacZYA-argF)U169 λ− | Invitrogen Corporation |
| TG1 | Δ(lac-proAB) thi supE hsdD5 (F′ traD36 proAB+ lacIqlacZΔM15) | 10 |
| RE707 | Hfr(PO45) thi Δ(ptsHI-crr) galP gyrA Δ(recA-slrR)306 slr301::Tn10 | 6 |
| RE777 | his leu ilv Δlac mglP ΔgalP Δ(recA-slrR)306 slr301::Tn10 | 6 |
a
The Δ(spo0E) mutation and the gntRK′-′lacZ fusion are associated with kanamycin and phleomycin resistance, respectively. The mini-Tn10 transposon utilized confers resistance to spectinomycin.
b
tf, the indicated chromosomal DNA was used to transform the indicated recipient strain.