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. 2023 Oct 1;37(19-20):865–882. doi: 10.1101/gad.350736.123

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© 2023 Hurd et al.; Published by Cold Spring Harbor Laboratory Press

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Figure 6. — Metabolic analyses of Rat1a and MCF10A cell lines overexpressing MYC WT or individual K-to-R mutants. (A,B) Rat1a cell line ECAR and OCR Seahorse analyses, respectively. (n.s.) Nonsignificant statistical difference (n = 5). (C) Cell viability measured by acid phosphatase assay after treatment with the mitochondrial pyruvate carrier inhibitor UK5099 for 72 h (n = 6). (D) Fraction of cellular OCR derived from glutamine oxidation (n = 8). (E) Viability of the cell lines (normalized to MYC WT cells) cultured for 72 h in medium with the indicated glutamine concentrations (n = 6). (F,G) MCF10A cell line ECAR and OCR Seahorse analyses, respectively, as above. The data (n = 5–8) were plotted as the means ± SD (A–E) or means ± SEM (F,G).