TABLE 1.
Distribution of Hsp60 epitopes and OmpS epitopes in typical sections of L. pneumophila SVir as detected by immunoelectron microscopy with various polyclonal rabbit immunoreagents
| Position | Distributiona of epitopes as labeled by:
|
|||||
|---|---|---|---|---|---|---|
| PAb
|
PAb + Hsp60
|
Anti-OmpS
|
||||
| Non-HSb | HSc | HS | % Inhibitiond | Non-HS | HS | |
| Cytoplasm | 11.1 ± 0.6 (26.2) | 6.2 ± 1.1 (22.4) | 2.8 (30.8) | 54.8 | 6.9 (14.6) | 10.0 (21.3) |
| Cyt. membr. | 6.6 ± 1.8 (15.6) | 5.0 ± 2.3 (18.0) | 1.5 (16.5) | 70.0 | 4.4 (9.3) | 4.7 (10.0) |
| Periplasm | 11.6 ± 1.3 (27.4) | 5.2 ± 2.1 (18.8) | 1.1 (12.1) | 78.9 | 16.6 (35.0) | 13.7 (29.2) |
| OM-surface | 13.0 ± 1.6 (30.7) | 11.3 ± 0.4 (40.8) | 3.7 (40.6) | 67.3 | 19.5 (41.1) | 18.5 (39.5) |
| Total | 42.3 ± 0.6 (100) | 27.7 ± 1.2 (100) | 9.1 (100) | 67.1 | 47.4 (100) | 46.9 (100) |
The distribution of gold particles standardized to the dimensions of the typical L. pneumophila section is shown in number of particles per cell compartment and as relative percent (in parentheses) with respect to the total number (100%) of particles per section. Cyt. membr., cytoplasmic membrane; OM-surface, outer membrane and surface; Non-HS, non-heat shocked (30°C); HS, heat shocked (42°C for 1 h). The background values for nonspecific labeling (irrelevant antibody controls) have been subtracted from the values shown for each condition.
Results are shown as means ± standard deviations of two independent labeling experiments. Each experiment included the analysis of 30 sections.
Results are shown as means ± standard deviations of three independent labeling experiments. Each of the experiments included the analysis of 30 sections.
% Inhibition, percent reduction of gold particles per compartment compared to that for HS labeled with PAb.