TABLE 3.
Distribution of Hsp60 epitopes in typical sections of the control species, E. coli PSH16, and B. pertussis F6321 as detected by immunoelectron microscopy with PAb or MAba
| Position | Distribution of epitopes as labeled by:
|
||||
|---|---|---|---|---|---|
| PAb
|
MAbb
|
||||
| B. pertussis non-HS |
E. coli PSH16
|
E. coli PSH16
|
|||
| Non-HSc | HSc | Non-HS | HS | ||
| Cytoplasm | 6.8 (37.4) | 26.5 ± 3.0 (83.9) | 62.9 ± 6.1 (85.8) | 4.80 (82.2) | 6.01 (90.4) |
| Cyt. membr. | 2.0 (11.0) | 2.5 ± 0.3 (7.9) | 4.4 ± 0.3 (6.0) | 0.30 (5.2) | 0.26 (3.9) |
| Periplasm | 4.8 (26.4) | 1.7 ± 1.1 (5.4) | 3.6 ± 0.9 (4.9) | 0.27 (4.6) | 0.16 (2.4) |
| OM-surface | 4.6 (25.2) | 0.9 ± 0.4 (2.8) | 2.4 ± 0.8 (3.3) | 0.47 (8.0) | 0.22 (3.3) |
| Total | 18.2 (100) | 31.6 ± 4.8 (100) | 73.3 ± 7.5 (100) | 5.84 (100) | 6.65 (100) |
a
For definitions and general organization, see footnote a to Table 1.
b
Only results from the protocol with an overnight labeling with the primary antibody are shown.
c
Results are shown as the means ± standard deviations of two (Non-HS) or three (HS) independent labeling experiments. Each experiment included the analysis of 30 sections.