TABLE 4.
Distribution of Hsp60 epitopes in typical sections of replicating L. pneumophila 2064 contained in endosomes of infected HeLa cells as detected by immunoelectron microscopy with PAb or MAba
| Position | Distribution of epitopes as labeled by:
|
|||
|---|---|---|---|---|
| PAbb | PAb + Hsp60 | % Inhibitionc | MAbd | |
| Cytoplasm | 4.2 ± 2.4 (18.5) | 1.2 (23.1) | 71.4 | 8.7 (50.0) |
| Cyt. membr. | 3.5 ± 1.3 (15.4) | 0.7 (13.5) | 80.0 | 3.3 (19.0) |
| Periplasm | 6.2 ± 0.1 (27.3) | 1.4 (26.9) | 77.4 | 1.9 (11.0) |
| OM-surface | 8.8 ± 2.0 (38.8) | 1.9 (36.5) | 78.4 | 3.5 (20.0) |
| Total | 22.7 ± 5.8 (100) | 5.2 (100) | 77.1 | 17.4 (100) |
a
For definitions and general organization, see footnote to Table 1.
b
Results are shown as means ± standard deviations of two independent labeling experiments. Each experiment included the analysis of 30 sections.
c
% Inhibition, percent reduction of gold particles per compartment labeled with PAb + Hsp60 compared to those labeled with PAb.
d
Only results from the protocol with an overnight labeling with the primary antibody are shown.