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. 2023 Nov 30;82(6):458–469. doi: 10.1097/FJC.0000000000001484

FIGURE 5.

FIGURE 5.

MiR-143-5p affects macrophage-mediated inflammation and MMP secretion through CCL20. A, Binding analysis of miR-143-5p and CCL20. B, Double luciferase reporter gene experiment. C, RT‒qPCR analysis of miR-143-5p transfection efficiency. D, Western blot analysis of CCL20 transfection efficiency. E, Analysis of MMP activity using a reagent kit. F, Western blot analysis of MMP9 and MMP2. G, Flow cytometric analysis of CD11b and CD163. H, Western blot analysis of M1 macrophage and M2 markers. I, ELISA analysis of inflammatory factors. The uncropped original western blot images are shown in Supplemental Digital Content 1 (see File 1, http://links.lww.com/JCVP/A991). MiR-143-5p mimic versus NC, miR-143-5p mimic +oe-CCL20 versus miR-143-5p mimic, *P ˂ 0.05, **P ˂ 0.01, ***P ˂ 0.001.