Fig. 3.

The integrator complex promotes biogenesis of both type I and type II piRNAs. a) piRNA precursor fold changes in indicated knockdowns relative to empty vector control knockdown. All piRNA precursors are compared to precursors originating from loci which most depend on snpc-4 for the accumulation of their mature sequences. Statistical analysis was performed using a two-tailed Mann–Whitney Wilcoxon test. For all boxplots, lines display median values, boxes display first and third quartiles, and whiskers display 5th and 95th percentiles. b) piRNA fold changes in ints-1 knockdown relative to empty vector control knockdown. Type I and type II piRNAs are compared separately. Statistical analysis was performed using a two-tailed Student's t-test against a null hypothesis that the fold change between treatment and control is 0. For all boxplots, lines display median values, boxes display first and third quartiles, and whiskers display 5th and 95th percentiles. c) GFP expression in the type I and type II piRNA reporters in indicated RNAi knockdowns. † indicates L1 to adult same generation RNAi treatment. Asterisks indicate significant reporter activation, defined as having at least 20% of worms screened showing activated GFP. Total worms screened shown below each bar. Error bars represent standard deviation of activation between replicate RNAi plates.