Fig. 6.

Multifaceted involvement of conserved and essential machinery work in concert with small RNA-mediated gene silencing in germ cells. A model depicting a role for factors identified in our screen. The Integrator complex promotes piRNA biogenesis, a subset of nuclear pore factors promotes CSR-1 localization and interface with the nucleolus, second step splicing factors ensure proper endogenous splicing occurs, and protein import machinery allow for the nuclear Argonaute HRDE-1 to translocate into the nucleus to transcriptionally silence target genes.