Fig. 4. Rab3b, Adarb2, and Nbl1 differentially respond to acute stress after ELA exposure.
(A) Violin plots illustrating the expression of selected top scoring genes determined by scRNA-seq, namely, Rab3b in the GABAergic neuron cluster, Adarb2 in the DG glutamatergic neuron cluster 1, and Nbl1 in the CA3 glutamatergic neuron cluster 1 and CA1 glutamatergic neuron cluster 1. (B) Quantification of the Rab3b mRNA expression signal in cells positive for the GABAergic neuron marker Slc32a1 in the CA3 vHPC region. (C) Quantification of the Adarb2 mRNA expression signal in cells positive for the glutamatergic neuron marker Slc17a1 in the DG vHPC region. (D) Quantification of the Nbl1 mRNA expression signal in cells positive for the glutamatergic neuron marker Slc17a1 in the CA3 vHPC region. Box plots represent the 25%, median, and 75% quartile; whiskers span minimum to maximum. Two-way ANOVA with Tukey post hoc test. *P < 0.05, ***P < 0.001, ****P < 0.0001.