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. 2023 Nov 20;5(6):fcad317. doi: 10.1093/braincomms/fcad317

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© The Author(s) 2023. Published by Oxford University Press on behalf of the Guarantors of Brain.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Quantification of total brain and cerebellar volume in uba5^ex1s/ex1s animals at 6 dpf. (A) Maximum intensity projections of whole brain from Tg (Huc:EGFP); uba5^+/+ and Tg (Huc:EGFP); uba5^ex1s/ex1s 6 dpf zebrafish. (B) Total brain and (C) cerebellar volume analyses showed no significant changes in uba5^ex1s/ex1s (brain volume n = 9, 4, 4, 8, 9; cerebellar volume n = 10, 4, 4, 8, 9) compared to uba5^+/+ (brain volume n = 9, 3, 3, 7, 10; cerebellar volume n = 8, 4, 3, 9, 10). Each dot represents an individual zebrafish. Data represents raw data for five biological replicates plotted with the mean ± SD predicted by the mixed model statistical test. Scale bar = 100 µm. n.s., non-significant; Rep, replicate.