Fig. 1.

Cellular response of Opi1-deficient cells to genotoxic and proteotoxic stress. a) Working model for how Opi1 represses transcription of target genes. b) Effect of deletion of genes involved in inositol metabolism in sensitivity to genotoxins. c) Cells lacking Opi1 show sensitivity to different genotoxins. d) Cells lacking Opi1 do not show sensitivity to proteotoxic stress agents. e) Growth rate of wild-type and opi1Δ cells determined by monitoring OD₆₀₀ over time. The data points (geometrical symbols) were fitted to a nonlinear regression curve using GraphPad Prism version 9.0.0 to analyze the growth kinetics. For b), c), and d), 4-fold serial dilutions were spotted on the media indicated in the figure, and plates were grown for 2–3 days at 30°C.