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. 2023 Jul 13;225(1):iyad130. doi: 10.1093/genetics/iyad130

Fig. 3.

Fig. 3.

Opi1 translocates to the nucleus after MMS treatment and is required to repress INO1 expression during genotoxic stress. a) and b) Effect of inositol availability and MMS treatment on Opi1-GFP localization. Cellular localization of Opi1-GFP was scored manually, and the means and standard deviations from 2 independent experiments are shown. c) Cells treated with MMS in medium lacking inositol are still viable. d) Effect of inositol availability and MMS treatment on INO1 expression by RT-qPCR. Relative values of mRNA are described as fold change relative to a control condition (wt + INO). For c), 4-fold serial dilutions were spotted on the media indicated in the figure, and plates were grown for 2–3 days at 30°C. For d), experiments were made with 3 biological replicates, and statistical significance of the differences observed was calculated using 1-way ANOVA with Tukey's multiple comparison test.