FIG. 3.

Southern hybridization of chromosomal DNAs from Yersinia and E. coli strains with the irp1 probe. The chromosomal DNA was digested with EcoRI, and the resulting fragments were separated on a 1% agarose gel prior to Southern blotting. Hybridization was performed with a DIG-labeled PCR probe generated with primers i8513 and i8730. Lane 1, Y. pestis KUMA; lane 2, Y. pestis KIM; lane 3, Y. pestis KIM Δpgm; lane 4, Y. pseudotuberculosis 346; lane 5, Y. pseudotuberculosis 201; lane 6, Y. pseudotuberculosis PB1; lane 7, Y. enterocolitica 8081; lane 8, Y. enterocolitica WA-CS; lane 9, Y. enterocolitica Y5.27; lane 10, Y. enterocolitica Y-96-C; lane 11, Y. enterocolitica Y-108-C; lane 12, E. coli Phi; lane 13; E. coli DH5α.