TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Genotype and/or phenotype | Reference(s) or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | endA1 hsdR17 (rK− mK+) supE44 thi-1 recA1 gyrArelA1 Δ(lacZYA-argF)U169 (φ80lacZΔM15) | 30 |
| Phi | Psts | 19 |
| S17-1 λ | pir+ tra+ | 54, 39 |
| Y. enterocolitica | ||
| WA-C | Plasmidless derivative of strain WA-134, serotype O8. Spontaneous Nalr mutant | 31 |
| WA-C irp2 | WA-C with pGPIRP2 inserted into irp2 gene | This study |
| WA-CS | Spontaneous Smr mutant derived from WA-C | This study |
| WA fyuA | fyuA mutant of WA-C, Pstr | 44 |
| WA-CS irp1::Kanr | Derivative of WA-CS, Nalr Smr Kmr | This study |
| 8081 | Serotype O8 | 42 |
| H1852 fyuA | Derivative of WA-C, fur fyuA mutant of WA-C | This study |
| Y5.27 | Clinical isolate, serotype O5.27 | 31 |
| Y-96-C | Clinical isolate, serotype O9 | 31 |
| Y-108-C | Clinical isolate, serotype O3 | 31 |
| Y. pseudotuberculosis | ||
| 346 | irp2+ serotype O3 | S. Aleksic |
| 201 | irp2 serotype O3 | S. Aleksic |
| PB1 | Serotype O1 | R. R. Brubaker |
| Y. pestis | ||
| KIM | Lcr− Pgm+ | R. R. Brubaker |
| KIM Δpgm | Lcr− ΔPgm | This study |
| KUMA | Lcr− Pgm+ | R. R. Brubaker |
| EV76 | Pesticin-producing strain | R. R. Brubaker |
| Plasmids | ||
| pLAFR2 | Tcr Mob+ | 25 |
| 17A11 | pLAFR2 carrying a 23-kb insert of WA-C chromosomal DNA, Tcr | 44 |
| pBluescript KS II | Cloning vector, Apr | Stratagene |
| pKAS 32 | Cloning vector with rpsL gene | 55 |
| pKAS-1SKan | pKAS 32 containing irp1 EcoRI-2 fragment with a kanamycin cassette introduced into SalI site | This study |
| pKAS-E1Kan | pKAS 32 containing EcoRI/SalI fragment of irp1 with a kanamycin cassette (without transcriptional terminator) inserted into the EcoRV site | This study |
| pGP704 | Cloning vector, Apr | 39 |
| pGP-CAT | Chloramphenicol cassette inserted into PstI site of pGP704, Cmr | 46 |
| pGPIRP2 | 600-bp PCR fragment of irp2 inserted into pGP-CAT vector | This study |
| pUC-4K | pUC vector containing kanamycin cassette from Tn903, Apr Kanr | 60 |
| pSB 315 | Containing kanamycin cassette without transcriptional terminator, Apr Kanr | 27 |
| pACYC 184 | Cloning vector, Cmr | 11 |
| pCJG3.3N | pACYC 184 with fyuA-gfp mut3 | This study |
| pGFP mut3 | pKS with cDNA of the mutated GFP under the lac promoter | 12 |
| SuperCos1 | Cloning vector lacking SalI restriction site, Apr Neor | Stratagene |