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. Author manuscript; available in PMC: 2024 Nov 8.
Published in final edited form as: J Am Chem Soc. 2023 Oct 2;145(44):24240–24248. doi: 10.1021/jacs.3c08674

Figure 4.

Figure 4.

Mechanical shear induces similar amyloid fibril aggregates at the FUS condensate surface. a,b. Sequentially acquired CRANAD-2 SMLM images and Cy3B SMdM D maps, and overlaid images, for FUS condensates subjected to 20 (a) and 200 (b) times of pipetting. Arrows in (a,b) point to extrusions and intrusions of the surface aggregates. c. Surface coverage of CRANAD-2 SMLM signal for FUS condensates pipetted 20 times. Open circles: Individual condensates. Filled circle and error bar: Average and standard deviation. d. Model: Enrichment of amyloid aggregates in the condensates and accumulation at the surface during aging.