TABLE 2.
Summary of measured and calculated cell cycle parameters for B. subtilis SG38 at different growth rates
| Cell cycle parameter | Culture medium
|
|||
|---|---|---|---|---|
| S | TS | CH | CHG | |
| Generation time (min) | 73 | 58 | 40 | 30 |
| Avg cell length (μm) | 2.2 | 3.1 | 3.6 | 4.5 |
| Avg cell width (μm)a | 0.81 (0.093) | 0.83 (0.077) | 0.86 (0.072) | 0.81 (0.075) |
| Avg DNA content/ cell (G)b | ||||
| Microscopic | 1.6 | 2.6 | 2.9c | 3.3 |
| Chemical | 1.5 | 2.3 | NDe | 3.1 |
| C period (min) | 58 | 53 | 50 | 52 |
| Birth | ||||
| Length (μm) | 1.6 | 2.2 | 2.6 | 3.0 |
| DNA content (G) | 1.3 | 1.7 | 2.0 | 2.3 |
| Initiation of DNA replication | ||||
| Length (μm) | 2.8 | 2.7 | 4.8 | 4.4 |
| Time (min) | 58 | 19 | 37 | 17 |
| DNA content (G) | 2 | 2 | 3.6 | 3.2 |
| Li (μm)f | 1.4 | 1.4 | 1.3 | 1.1 |
| Termination of DNA replication | ||||
| Length (μm) | 2.4 | 2.6 | 2.9 | 3.7 |
| Time (min) | 43 | 14 | 7 | 9 |
| DNA content (G) | 2 | 2 | 2.4 | 2.8 |
| D* period (min) | 30 | 34 | 34 | 21 |
| Nucleoid separation | ||||
| Length (μm) | 3.0 | 3.0 | 3.0 | 3.7 |
| Time (min) | 63 | 26 | 9 | 10 |
| DNA content (G) | 2.2 | 2.3 | 2.5 | 2.9 |
| Nucleoid bilobation | ||||
| Length (μm) | 2.6 | 2.3 | 4.1d | 4.8d |
| Time (min) | 49 | 2.6 | 26d | 21d |
| DNA content (G) | 2.0 | 1.8 | 3.2d | 3.5d |
Values in parentheses are standard deviations.
G, the DNA content of a single completed chromosome.
Data from reference 15.
Transition from binucleate to binucleate bilobed state.
ND, not done.
Cell length per copy of oriC.