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. 1998 Feb;180(3):547–555. doi: 10.1128/jb.180.3.547-555.1998

TABLE 2.

Summary of measured and calculated cell cycle parameters for B. subtilis SG38 at different growth rates

Cell cycle parameter Culture medium
S TS CH CHG
Generation time (min) 73 58 40 30
Avg cell length (μm) 2.2 3.1 3.6 4.5
Avg cell width (μm)a 0.81 (0.093) 0.83 (0.077) 0.86 (0.072) 0.81 (0.075)
Avg DNA content/ cell (G)b
 Microscopic 1.6 2.6 2.9c 3.3
 Chemical 1.5 2.3 NDe 3.1
C period (min) 58 53 50 52
Birth
 Length (μm) 1.6 2.2 2.6 3.0
 DNA content (G) 1.3 1.7 2.0 2.3
Initiation of DNA replication
 Length (μm) 2.8 2.7 4.8 4.4
 Time (min) 58 19 37 17
 DNA content (G) 2 2 3.6 3.2
Li (μm)f 1.4 1.4 1.3 1.1
Termination of DNA replication
 Length (μm) 2.4 2.6 2.9 3.7
 Time (min) 43 14 7 9
 DNA content (G) 2 2 2.4 2.8
D* period (min) 30 34 34 21
Nucleoid separation
 Length (μm) 3.0 3.0 3.0 3.7
 Time (min) 63 26 9 10
 DNA content (G) 2.2 2.3 2.5 2.9
Nucleoid bilobation
 Length (μm) 2.6 2.3 4.1d 4.8d
 Time (min) 49 2.6 26d 21d
 DNA content (G) 2.0 1.8 3.2d 3.5d
a

Values in parentheses are standard deviations. 

b

G, the DNA content of a single completed chromosome. 

c

Data from reference 15

d

Transition from binucleate to binucleate bilobed state. 

e

ND, not done. 

f

Cell length per copy of oriC.