TABLE 3.
Calculation of the DNA replication (C) period
| Medium | Relative hybridization (cpm) witha:
|
Avg ratio (R) of oriC/terC | log R/log 2 (N)b | Generation time (T) (min) | C period (N × T) (min)c | |||
|---|---|---|---|---|---|---|---|---|
|
oriC marker
|
terC marker
|
|||||||
| spoIIIJ (360°) | dnaA (0°) | rtp (178°) | cge (181°) | |||||
| S | 0.45 (407) | 0.43 (810) | 0.29 (189) | 0.22 (351) | 1.73 | 0.79 | 73 | 58 ± 13% |
| TS | 1.4 (1,282) | 1.1 (1,998) | 0.72 (463) | 0.63 (1,179) | 1.9 | 0.92 | 58 | 53 ± 11% |
| CH | 3.9 (3,587) | 4.6 (5,473) | 1.7 (1,143) | 1.9 (5,492) | 2.4 | 1.26 | 40 | 50 ± 10% |
| CHG | 1.7 (1,545) | 1.3 (2,460) | 0.5 (317) | 0.4 (757) | 3.3 | 1.72 | 30 | 52 ± 11% |
a
Hybridization of the probe to the test DNA, relative to that of the control DNA from sporlets of strain 1.5. Averages of three independent experiments are given. Data in parentheses represent the average counts per minute for the test DNA samples.
b
From reference 46.
c
Percentages are standard deviations.