Table 3.

Physicochemical characteristics of the prepared SLNs at zero time and after storage for 3 months at 4 °C

Code	Particle size (nm)		PDI		zeta potential (mV)		%Entrapment efficiency
	Zero time	3 months	Zero time	3 months	Zero time	3 months	Zero time		3 months
							MTX	RH	MTX	RH
Placebo-SLNs	136.53 ± 7.68	141.24 ± 5.31	0.36 ± 0.05	0.29 ± 0.08	− 44.60 ± 1.04	− 43.24 ± 2.10	–	–	–	–
MTX-SLNs	182.33 ± 2.83	186.52 ± 3.14	0.30 ± 0.01	0.34 ± 0.04	− 46.66 ± 0.75	− 44.97 ± 1.56	90.45 ± 2.14	–	88.78 ± 4.26	–
RH-SLNs	150.67 ± 6.96	153.14 ± 7.21	0.32 ± 0.08	0.36 ± 0.02	− 43.50 ± 2.48	− 45.23 ± 3.25	–	95.62 ± 1.07	–	94.87 ± 0.74
RH-MTX SLNs	192.37 ± 3.07	196.94 ± 4.56	0.37 ± 0.02	0.39 ± 0.01	− 47.43 ± 1.90	− 45.14 ± 2.64	88.21 ± 3.10	93.02 ± 0.95	87.85 ± 2.49	92.35 ± 1.25

Results are represented as mean ± SD (n = 3)