Table 2.
Optimization of host and vector systems in the expression of lipase EMP48-D
| Host | Vector | Clear zone indexa | Pellet activity (U/mg) |
|---|---|---|---|
| Wild-type | n/a | 1.25 | 17.02 ± 3.01 |
| E. coli DH5α | pGEM-T Easy | n/a | 2.11 ± 1.09 |
| pUC-19 | n/a | 0.93 ± 0.07 | |
| E. coli BL21 (DE3) | pLysS + pET15-b | 1.0 | 15.02 ± 2.33 |
| pET15-b | 0.95 | 14.47 ± 1.99 | |
| E. coli BL21 with Origami variants | pG-KJE8 + pET15-b | 0.75 | 10.33 ± 2.88 |
| pGro7 + pET15-b | 0.45 | 7.65 ± 1.56 | |
| pKJE7 + pET15-b | 0.7 | 10.02 ± 0.98 | |
| pG-Tf2 + pET15-b | 0.65 | 8.57 ± 2.55 | |
| pTf16 + pET15-b | 0.4 | 5.35 ± 1.62 |
aThe clear zone index was measured after 24 h of incubation on tributyrin agar and was calculated as the ratio of the diameter of the clear zones to the diameter of colonies
The activity was expressed as the mean ± standard deviation