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. 2023 Aug 18;44(12):2455–2468. doi: 10.1038/s41401-023-01140-4

Fig. 6. RVG-cluster 3-Exos exert antifibrotic activity by targeting multiple genes.

Fig. 6

a Heatmap showing differentially expressed targets identified by RNA-seq in the TECs treated with RVG-vector-Exos (1), RVG-miR-23a/27a/26a-Exos (2), RVG-miR-23a/27a-Exos (3) or RVG-miR-26a-Exos (4) under HG conditions. b Intersection analysis of the RNA-seq, TargetScan and miRDB databases. c qRT‒PCR verified the relative expression levels of Lpp, Zbtb20 and Klhl42 in different groups in vivo. n = 7 per group. d Western blot analysis of Lpp, Zbtb20 and Klhl42 in vivo. n = 7 per group. e Immunohistochemistry confirmed the location of Lpp, Zbtb20 and Klhl42 in the kidney and the relative expression in vivo. Scale bars, 50 μm. f Luciferase reporter assays identified Lpp as a target in common of miR-23a-3p and miR-26a-5p, Klhl42 as a target of miR-26a-5p and Zbtb20 as a target of miR-27a-3p. n = 3 per group. **P < 0.01, ***P < 0.001. ns, no significant difference; NC, negative control; WT, wild type; MUT, mutant; Lpp, lipoma-preferred partner; Zbtb20, Zinc finger and BTB domain containing 20; Klhl42, Kelch-like protein 42.