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. 2023 Aug 14;44(12):2469–2478. doi: 10.1038/s41401-023-01143-1

Fig. 7. Fraxinellone interfered with the HSP47-collagen complex by directly binding to HSP47.

Fig. 7

a Molecular docking analysis of fraxinellone and the HSP47-collagen complex. b Proteins were isolated and immunoprecipitated with an antibody against GFP. c The effect of fraxinellone and Col003 on the thermal stability of human HSP47 WT. d The binding ability between human HSP47 WT and fraxinellone or Col003. e Dose response curves for a gradient dilution of fraxinellone binding to HSP47-WT were measured by SPR. f The effect of fraxinellone and Col003 on the thermal stability of the human HSP47 Mut. g The binding ability between human HSP47 Mut and fraxinellone or Col003. h Dose response curves for a gradient dilution of fraxinellone binding to HSP47-Mut were measured by SPR. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01 vs. DMSO.