TABLE 1.
Strains and plasmids used in this work
| Strain or plasmida | Relevant genotype or characteristics | Reference or source |
|---|---|---|
| K. aerogenes | ||
| MK9682 | gltB200 ntr-45 | 4 |
| KB958 | hutC515 nac-1 | 7 |
| MK1 | Prototroph | B. Magasanik |
| KC1422 | hutC515 dadA1 (Mu cts hP1 1) | This laboratory |
| KC2001 | gltB200 ntr-45 ntrC5::Tn5-131 | 25 |
| KC2004 | hutC515 Δ[bla]-2 nac-203::Tn5-131 | This laboratory |
| KC2569 | hutC515 dadA1 nas-3::Tn5-131 | 5 |
| KC2616 | hutC515 Δ[bla]-2 crp-91::Tn5-131 | This laboratory |
| KC2668 | hutC515 Δ[bla]-2 dadA1 | This laboratory |
| KC2725 | nac-203::Tn5-131 in KC2668 | This laboratory |
| KC2863b | gdh-3 in KC2668 | This laboratory |
| KC3345c | hutC515 nac-203::Tn5-131 | P1 (KC958) × KC2004 |
| KC3346d | hutC515 Δ[bla]-2 | P1 (KC3345) × KC2668 |
| KC3821 | KC3346/pCB888 | This work |
| KC3847 | KC3346/pPC36 | This work |
| KC3848 | KC3847/pCB888 | This work |
| KC3902 | KC2668/pCB515 | This laboratory |
| KC3914 | hutC515 (Mu cts hP1 1)/pEG5005 | This work |
| KC4080 | nas-3::Tn5-131 in KC3346 | This work |
| KC4469e | hutC515 Δ[bla]-2 crp-91::Tn5-131 | P1 (KC2616) × KC3346 |
| E. coli | ||
| W3110 | Prototroph | R. G. Matthews |
| EB3364 | nac-28 in W3110 | 32 |
| EB3846 | W3110/pPC36 | This work |
| Plasmids | ||
| pBS SK− | High-copy-number cloning plasmid, Apr | Stratagene |
| pEG5005 | Carries Mu d5005, Apr Kmr | 20 |
| pRJ800 | Promoterless lacZ fusion plasmid, Apr | 2 |
| pPC36 | Low-copy-number tacp::nac fusion, Smr Spr | S. Maloy |
| pCB515 | Low-copy-number gdhA, Smr Spr | This laboratory |
| pCB752 | K. aerogenes dadAB operon in pBS SK− | This work |
| pCB888 | K. aerogenes dadAp in pRJ800 | This work |
| pCB889 | E. coli dadAp in pRJ800 | This work |
| pCB925 | Mu d5005::dadAB | This work |
a
All K. aerogenes strains listed here are derived from strain W70; all E. coli strains are derived from strain K-12.
b
gdh-3 in a mutation that results in an approximately threefold increase in GDH activity when cells are grown in glucose-ammonium minimal medium.
c
Transduction of strain KC2004 to Dad+ (growth on alanine as the sole carbon and nitrogen source), using phage P1 grown on strain KC958.
d
Transduction of strain KC2668 to Dad+ (growth on alanine as the sole carbon and nitrogen source), using phage P1 grown on strain KC3345.
e
Transduction of strain KC3346 to crp-91::Tn5-131 (tetracycline resistance), using phage P1 grown on strain KC2616.