TABLE 4.
Derepression of GS by alanine in K. aerogenes MK1
Growth mediuma | GS sp actb |
---|---|
GNgln | 149 ± 44 |
GN | 476 ± 135 |
GNala | 947 ± 98 |
Ggln | |
0.2% | 1,452 ± 37 |
0.04% | 2,130 ± 233 |
Growth medium was W4 salts supplemented with 0.4% glucose (G), 0.2% (NH4)2SO4 (N), 0.2% l-alanine (ala), and l-glutamine (gln) as indicated.
Detergent-treated cells were assayed for the total amount of GS present in the transferase assay (6). Results are the average and standard deviation of at least three independent assays.