TABLE 5.
Regulation of d-amino acid dehydrogenase activity in E. coli
| Strain | Relevant genotype | Growth mediuma | d-Amino acid dehydrogenase sp actb |
|---|---|---|---|
| W3110 | GN | 19 ± 1 | |
| GNala | 250 ± 26 | ||
| EB3364 | nac-28 | GN | 21 ± 8 |
| GNala | 325 ± 25 | ||
| EB3846 | pPC36c | GN | 20 ± 9 |
| GNIPTG | 30 ± 12 |
a
Growth medium was W4 salts supplemented with 0.4% glucose (G), 0.2% (NH4)2SO4 (N), 0.05% l-alanine (ala), and 1 mM IPTG (to induce nac expression from the tac promoter [pPC36]) as indicated.
b
Average and standard deviation of at least three independent assays.
c
Contains nac driven by the IPTG-inducible tac promoter.