Table 2.
Key reagents for drug sensitivity screens.
| Product | Description | Supplier | Catalogue number |
|---|---|---|---|
| Lymphoprep | Density gradient medium | PROGEN | 1856-4 |
| Lysing Buffer BD Pharm ™ | RBC lysis | BD Biosciences | 555899 |
| Mononuclear Cell Medium | Medium for short maintenance of mononuclear cells | Promo Cell | C-28030 |
| RPMI1640 Cell Medium with L-Glutamine | Medium for culturing of mononuclear cells | VWR | 392-0428 |
| 384-well plate | Sterile 384 cell culture plates white, opaque bottom | Greiner Bio-one/BioNordika | 781080 |
| 1536-well plate | Sterile 1536 cell culture plates white, opaque bottom | Corning Life Sciences | CLS3727 |
| Breathe-Easy® sealing membrane | Membrane permeable to O2/CO2, to limit H2O evaporation | Merck life science (Sigma) | Z380059-1PAK |
| CellTiter-Glo 2.0 Assay | Luminescent cell viability assay | Promega | G9243 |