Table 1.

Description of samples used to acquire RNA-seq data, with the number of reads retained or removed at various stages of preprocessing.

Sample ID	Habitat	Biological Replicate	Raw Reads	After kmer correction	The final number of reads	Retained reads [%]
Np_Lo_13	control	1	147,650,836	129,641,042	122,012,486	82.6
Np_Lo_14	control	2	121,309,051	106,229,479	105,798,037	87.2
Np_Lo_15	control	3	132,859,384	115,160,311	114,498,350	86.2
Np_Ze_12	metal-enriched	1	140,954,632	122,472,560	122,138,223	86.7
Np_Ze_14	metal-enriched	2	141,952,259	124,684,580	124,450,757	87.7
Np_Ze_15	metal-enriched	3	117,208,939	102,304,432	100,420,524	85.7
Total			801,935,101	700,492,404	689,318,377	86.0

Leaves of Noccaea praecox (Np) were collected either on the control site (Lo) or metal-enriched site (Ze).