TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genotype or phenotypea | Reference(s) or source |
|---|---|---|
| Strain | ||
| S. typhimurium | ||
| MS1582 | leuA414(Am) Fels−supE40 ataA::(P22 sieA44 16-amH1455 tpfr49) | 9 |
| MST1762 | MS1868/pMS421 | P. Youderian |
| MS1868 | leuA414(Am) Fels−hsdSB | 9, 27 |
| MS1883 | leuA414(Am) Fels−hsdSB supE40 | 9 |
| TH564 | leuA414(Am) Fels−supE40 ataA::(P22 sieA44 Ap7 [Tn1] tpfr184 [mnt-al] 9−att+) | 12 |
| MST2778 | MS1868/pPC36 | This study |
| MST2779 | TH564/pPC36 | This study |
| MST2780 | TH564/pGW1700/pPC36 | This study |
| E. coli DH1 | thi-1 supE44 hsdR17(r− m+) endA1 recA1 relA gyr-96 | 11 |
| Plasmid | ||
| pEC205.1 | Ptac-nac+ (pBR322) | 24 |
| pGW1700 | Tetr AmprmucAB (pBR322) | 20 |
| pMS421 | Strr SpcrlacIq (pSC101) | 9 |
| pKC7 | KanrputK+ (K. aerogenes) | 4 |
| pKC9 | Ampr (subcloned pKC7 HindIII[283]-HindIII[621] into pTZ18U HindIII) | 4 |
| pPC6 | AmprputS+ (S. typhimurium) | 10 |
| pPC36 | Spcr Strrnac+ (pMS421) | This study |
| pPC37 | Tetr Ampr (subcloned pKC9 DraI[381]-SmaI[436] into pPY190 SmaI) | This study |
| pPC38 | Tetr Ampr (subcloned pKC9 SmaI[436]-DraI[381] into pPY190 SmaI) | This study |
| pPC39 | Tetr Ampr (subcloned pKC9 SmaI[436]-HincII[406] into pPY190 SmaI) | This study |
| pPC40 | Tetr Ampr (subcloned pKC9 HincII[406]-SmaI[436] into pPY190 SmaI) | This study |
| pPC41 | Ampr (subcloned pKC7 between putK oligonucleotide 1 and putK oligonucleotide 6 into pTZ18U SmaI) | This study |
| pPC42 | Ampr (subcloned pKC9 HindIII[283]-SmaI[436] into pTZ18U SmaI) | This study |
| pPC43 | Ampr (subcloned pKC9 SmaI[436]-HindIII[621] into pTZ18U SmaI) | This study |
| pPC44 | Ampr (subcloned pKC9 HindIII[283]-HincII[406] into pTZ18U SmaI) | This study |
| pPC45 | Ampr (subcloned pKC9 HincII[406]-HindIII[283] into pTZ18U SmaI) | This study |
| pPC46 | Ampr (subcloned pKC9 HindIII[283]-DraI[381] into pTZ18U SmaI) | This study |
| pPC47 | Ampr (subcloned pKC9 DraI[381]-HindIII[283] into pTZ18U SmaI) | This study |
| pPY190 | Ampr Tetr P22 mnt Pant SmaI-XmaI ant′ (pBR322) | 2 |
| pTZ18U | Ampr | 18 |
a
Genetic nomenclature for bacterial genes is as described in Sanderson and Roth (22). Numbers in brackets indicate the map distances in base pairs of the restriction sites from the transcriptional start site of the putP gene.